Restriction Landmark Genomic Scanning Analysis of Breast Cancer for Detection of Novel Amplicons
Abstract
Genomic amplification is believed to be a pivotal event in the origin and progression of a variety of human tumors including breast cancers. Recently, a technical innovation potentially suitable for detecting candidate novel amplifications in tumors, called restriction landmark genomic scanning, has been described by Hyashizaki and co-workers. This is a two-dimensional DNA restriction analysis technique by which a highly reproducible constellation of -2,000 spots (each corresponding to a specific genetic locus) can be surveyed. As spot intensity is proportional to gene copy number in this system, amplified loci in tumor-derived DNAs are readily detected. Using this approach, several investigators including ourselves have demonstrated both previously identified and novel amplicons in several tumor types. A derivative technology allows direct cloning of amplified sequences from DNA isolated from excised spots. I propose here to study up to 20 breast tumor specimens in this way. Candidate novel amplicons identified will be cloned; initial characterization of these amplicons will entail their genomic localization using fluorescence in situ hybridization.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1997
- Accession Number
- ADA339179
Entities
People
- Eric H. Radany
Organizations
- University of Michigan