Biotherapy of Breast Cancer with EGF-Genistein.
Abstract
Human epidermal growth factor (EGF) is a 53 amino acid, single-chain polypeptide (Mr 6216 daltons), which exerts biologic effects by binding to a specific 170 kDa cell membrane epidermal growth factor receptor (EGF-R/ErbB-1). Many types of cancer cells display enhanced EGF-R expression on their cell surface membranes. Enhanced expression of the EGF-R on cancer cells has been associated with excessive proliferation and metastasis. Examples include breast cancer, prostate cancer, lung cancer, head and neck cancer, bladder cancer, melanoma, and brain tumors. In breast cancer, expression of the EOF-R is a significant and independent indicator for recurrence and poor relapse-free survival. The human EOF-R consists of an extracellular domain with a high cysteine content and N-linked glycosylation, a single transmembrane domain, and a cytoplasmic domain with protein tyrosine kinase (PTK) activity. Binding of EGF to the EUF-R/ErbB-l results in receptor dimerization with itself or other members of the Erb-B (subtype I) transmembrane PTK family (e.g., Erb-B2, Erb-B3), resulting in activation with autophosphorylation of the PTK domain.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1997
- Accession Number
- ADA340572
Entities
People
- Roland Gunther
Organizations
- University of Minnesota