A Novel Model System to Examine Agents Used in Breast Cancer Therapy.

Abstract

We have previously described the isolation and characterization of an intact multiprotein complex for DNA synthesis, designated the DNA synthesome, from human breast cancer cells and biopsied human breast tumor tissue. In the presence of the viral large T-antigen and simian virus 40 (SV4O) origin sequences, the DNA synthesome fully supports semiconservative SV4O DNA replication in vitro. We have shown that the proteins and enzymes constituting the breast cell DNA synthesome include: DNA polymerases alpha, delta and epsilon, DNA primase, proliferating cell nuclear antigen (PCNA), replication factor C (RF-C) and replication protein A (RP-A).

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA340656

Entities

People

  • Jennifer Coll
  • Linda H. Malkas

Organizations

  • University of Maryland School of Medicine

Tags

DTIC Thesaurus Topics

  • Antigens
  • Antineoplastic Agents
  • Biochemistry
  • Biological Sciences
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Molecular Biology
  • Molecules
  • Multiprotein Complexes
  • Neoplasms
  • Oncology
  • Pharmacology
  • Phosphodiesterases
  • Protein-Protein Interactions
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics