Identification and Localization of Genes Which Restore Senescence in Breast Cancer Cells
Abstract
The objective of this project is to identify and map genes capable of restoring senescence to immortal breast cancer cells by functional complementation. The methods used to achieve this goal are cell X cell hybridizations and microcell fusions between the tumor and normal cells containing gpt tagged single normal human chromosomes. Cell X cell hybridizations between breast tumor cells and normal cell lines containing gpt tagged human chromosomes 2, 3, 6 and 9 resulted in senesced hybrid clones whereas hybrid clones containing human chromosome 4 did not senesce. Introduction of individual gpt tagged normal human chromosomes 6, 9 and 16, by using microcell mediated chromosome transfer technique, showed characteristic morphological and growth properties typical to senescence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA340659
Entities
People
- Deepthi Reddy
Organizations
- Temple University