Integrin Regulation of Apoptosis in Breast Cancer.
Abstract
Using homology PCR and RACE, I cloned a novel gene from breast carcinoma cell line MCF-7. This ADAM protein, which is designated as ADAM::, has the highest homology to the monkey fertilln I. The protein sequence reveals that it constitutes the following domains characteristic of the ADAM proteins: a prodomain that contains the putative cysteine-switch cysteine residue, an active metalloprotease domain as evidenced by the existence of the zinc-binding moti?, a disintegrin domain comprising highly conserved cysteine arrangement paftern found in all soluble snake venom disintegrins and previously identified ADAMs, a cysteine-rich domain that contains a division peptide, a well defined EGF domain and a putative transmembrane domain. Most importantly, ADANk is not found in highly and moderately metastatic breast carcinoma cell lines MDA-MB-43S and MDA-MB-23 1. Experiments will be focused on the interaction between the disintegrin domain and integrin receptors. The ultimate goal is to define the role of ADAMx in tumor metastasis and apoptosis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA340730
Entities
People
- Dana Hu
Organizations
- Sanford Burnham Prebys Medical Discovery Institute