Protein Kinases in Human Breast Carcinoma
Abstract
The central aims of this project are to characterize novel kinases in breast cancer biology. During this first year of the award, we have centered our work on the Rak tyrosine kinase. Rak is a nuclear tyrosine kinase identified from breast cancer cells, which is a potent growth suppressor gene. We have demonstrated Rak's growth inhibitory effects in several different breast cancer cell lines. We have also explored the mechanism of the growth inhibition to determine whether this is related to inhibition of the CDC2 kinase, and which specific sequences of Rak are required for the growth inhibition. Our current work is focusing on characterization of an inducible system of Rak expression in breast cancer cell lines to further analyze the mechanism behind this growth arrest. We also are developing monoclonal antibodies to Rak to study both the levels of Rak expression and its nuclear localization in large samples of human breast cancers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA340944
Entities
People
- William G. Cance
Organizations
- University of North Carolina at Chapel Hill