Line-1 Retrotransposons as Mutagens in Human Breast Cancer.
Abstract
The human LINE-1 retrotransposon (L1Hs) is known to cause mutations by inserting into genes and inactivating them. The element is expressed in many breast tumors and breast mor cell lines, suggesting that L1Hs-induced mutations may play some role in this malignancy. To test this hypothesis we plan to place a 'tagged' LlHs element in non- malignant breast epithelial cells and let it transpose. If the cells become malignant, we can isolate the gene(s) into which the element has transposed by using the unique tag. Previously we tested a number of plasmid and retroviral vectors in several cell lines without success. We have recently found that a non-integrating 'tagged' LlHs vector may be useful if we can succeed in increasing the number of retrotransposition events/transfected cell population. In addition, we are using the expression vector pIRESineo (producing a bicistronic niRNA) to express L1Hs-encoded proteins in transfected MCFiOA cells. This system will be useful to test the effects of expression/overexpression of L1Hs-encoded proteins in non-malignant cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1997
- Accession Number
- ADA341479
Entities
People
- Thomas G. Fanning
Organizations
- Armed Forces Institute of Pathology