Studies on Human Breast Cancer and Transforming Growth Factor-beta Application for a Career Development Award.
Abstract
This is a career development award. The primary focus of the research is the expression and function of TGP-beta by breast cancer cells. For established tumors, overexpression of TGF-beta may result in increased in vivo tumor growth and metastatic spread. The first objective involved the analysis of resected breast cancer specimens, with the goal of confirming our prior work correlating clinical outcome with TGF-beta expression levels in tumors. Our analysis of a larger sample size of a uniform patient population has negated that observation. The second objective of the grant was to evaluate the impact of TGF-beta on the tumorigenic and metastatic potential of human breast cancer cell lines in nude mice. The first animal experiment suggests that overexpression of TGF-beta does not increase the tumorigenicity of the cells. The third research objective is the analysis of the regulation of TGF-beta expression in breast cancer cells. We have determined that the TGF-beta1 locus is not amplified in a significant proportion of breast cancers. In other experiments, we have detected a potential role of CpG methylation in the vicinity of the breast cancer specific TGF-beta3 promoter. This is a career development award. The primary focus of the research is the expression and function of TGP% by breast cancer cells. For established tumors, overexpression of TGF- 13 may result in increased in vivo tumor growth and metastatic spread. The first objective involved the analysis of resected breast cancer specimens, with the goal of confirming our prior work correlating clinical outcome with TGF-13 expression levels in tumors. Our analysis of a larger sample size of a uniform patient population has negated that observation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1997
- Accession Number
- ADA341483
Entities
People
- Bradley A. Arrick
Organizations
- Dartmouth College