Purification and Analysis of a Recombinant Human Anti-Cholera Toxin B Antibody.

Abstract

A large semi-synthetic recombinant antibody library was constructed based on a human anti-tetanus library. The library was used to isolate a human anti-cholera toxin B antibody cidne, which is expressed in XL-1 Blue E. Coli bacteria. The clone, B-27 cholera Fab, was expressed in small scale and purified by a two column procedure, which uses the histidine affinity tag, which was engineered into the protein. The purified cholera Fab was tested by ELISA to confirm specificity and by surface plasmon resonance to determine affinity binding constants.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1998
Accession Number
ADA341970

Entities

People

  • Darrel E. Menking
  • Jessica Dang
  • Michael J. Gostomski
  • Peter A. Emanuel
  • Suzanne Kracke

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Bacteriophages
  • Biosensors
  • Cell Line
  • Cells
  • Chemistry
  • Detection
  • Detectors
  • Genetic Engineering
  • Histidine
  • Production
  • Proteins
  • Resonance
  • Standards
  • Surface Plasmon Resonance
  • Surface Plasmons

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry