Characterization of the Cellular Stress Response Induced by Exposure to Organic Compounds.
Abstract
Experiments were conducted for this project to determine if various characteristics of the cellular stress response among tissues could serve as the basis for developing a suite of biomarkers that: (1) identify vulnerable target tissues; (2) delineate the cellular manifestations of toxicity; and (3) screen for exposure to specific classes of organic contaminants. Relationships were examined, at the cellular level, between accumulation and localization of stress proteins (stress-7O, chaperonin6O, and ubiquitin) and alterations in subcellular compartments using organic compounds with well characterized proteotoxic and genotoxic effects. These. objectives were accomplished using the following strategies: 1. Well characterized toxins were used to test the hypothesis that the induction and/or subcellular localization of stress-7O, and chaperonin, and ubiquitin, reflects the intercellular sites of toxicity of compounds with established mechanisms of toxicity; 2. Induction and subcellular localization of stress-7O, chaperonin, and ubiquitin by novel compounds were compared with the cellular manifestations of toxicity, 3. The pattern of accumulation of stress proteins, as a result of exposure to these novel compounds, was characterized: 4. Determined if the above compounds results in unique tissue and subcellular patterns of stress protein accumulation; 5. Determined is the tissue and subcellular patterns of stress protein accumulation could be used as "signatures" to distinguish compounds or classes of compounds from one another.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA343307
Entities
People
- Brenda Sanders
Organizations
- California State University, Long Beach