Taxol and LPS Modulation of c-kit and nm23 Expression in Macrophages and Normal vs. Malignanat Breast Cancer Cell Lines.
Abstract
Taxol is a microtubule poison that has been used successfully in refractory breast cancer. Apart from its well characterized anti-mitotic effects, Taxol shares with bacterial lipopoly- saccharide (LPS) the capacity to elicit microtubule-independent, intracellular signaling pathways in murine macrophages that activate a serine/threonine and tyrosine kinase cascade that leads to expression of many genes. This IDEA grant proposed to test the ability of Taxol to up-regulate expression of two genes, nm23 and c-kit, whose expression is down-regulated in advanced, metastatic breast cancer. In addition, modulation of adrenomedulin (AM), a gene expressed by both cancer cells and macrophages, has been examined. To date, we have: (1) optimized conditions for the detection of nm23, c-kit, and AM mRNA by reverse transcriptase polymerase chain reaction (RT-PCR) in murine cells, (2) demonstrated differential expression of rnn23 mRNA in two melanoma cell lines (TK1735 2-4 and the nm23 transfectant, TK1735 4- 6), and (3) demonstrated that both LPS and Taxol strongly up-regulate expression of AM, and to a lesser extent, c-kit and nm23, in LPS-responsive murine macrophages. We are now ready to move forward with our analysis of breast cancer cell lines as originally proposed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA343744
Entities
People
- Stefanie N. Vogel
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine