Role of Bc1-2 in Breast Cancer Progression.
Abstract
The anti-apoptotic gene bcl-2 is frequently overexpressed in many human tumors including invasive breast cancer. In vitro studies clearly demonstrate that the bcl-2 gene product prevents apoptosis following a variety of stimuli including radiation, hyperthermia, growth factor withdrawal and chemotherapeutic drugs. However, high levels of the bcl-2 expression have shown a positive correlation in clinicopathological studies, such as tumor grade and better response to hormonal treatment and chemotherapy. It is now critical to investigate the in vivo function of bcl-2 in human cells to elucidate the contradiction between clinical studies and in vitro studies. We have investigated the roles of bcl-2 in breast cancer development using MCF10A in vitro model and MCF10AT in vivo model. Here, we report that bcl-2 deregulates a G1/S checkpoint in MCF10A cells through upregulation of G1 cyclin activities, suggesting a role of bcl-2 as an oncogene. We then show that bcl-2 induces transformed phenotype in MCF10AT cells as determined by a soft agar assay. Bcl-2 expressing MCF10AT cells will be utilized for in vivo studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA343755
Entities
People
- Hyeong-reh Kim
Organizations
- Wayne State University