Breast Cancer Cell Metabolism Studied by MRS.
Abstract
It is proposed to study the metabolism of intact breast cancer cells using non-invasive magnetic resonance spectroscopy (MRS) methods. Magnetic resonance imaging (MRI) has been shown to have some value in assessing breast lumps, but lacks specificity. MRS can in principle provide this specificity. In order to evaluate the potential of MRS, systematic studies will be carried out on cell lines selected for the progressive estrogen independent phenotype and the effects of hormones and tamoxifen. The cell studies will provide basic information on the metabolic profile of different BC cell lines in the progression from estrogen and drug sensitive to estrogen independent and drug resistant phenotypes. Cells are embedded in gels and are examined in the MR spectrometer while undergoing perfusion. Previous studies have mainly used carbohydrate (agarose) gels. But, based on preliminary results, we will use a protein gel (Matrigel) for which the cells have surface receptors, so that their metabolism can be monitored while they are proliferating. This allows the effects not only of different phenotypes, but also of hormones, and antiestrogens such as tamoxifen, as well as certain drugs and drug combinations to be evaluated. Both 31P and proton MR methods will be applied. The results will be of significance not only to understanding the fundamental biological processes involved, but also to the observation of in vivo MRS of breast lumps.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA343795
Entities
People
- Jack S. Cohen
- Raymond Woosley
Organizations
- Georgetown University