Factors Modulating Estrogen Receptor Activity

Abstract

The goal of this project is to elucidate the mechanism of estrogen receptor (ER) action through the identification of Proteins involved in ER signaling using a genetic approach. We have utilized the phenotype of a mutant ER (G4O0V ER), deficient in its ability to bind hormone, as the basis for a dosage suppression screen in yeast. Screening a high-copy yeast genomic library, we have isolated a gene that dramatically suppresses the phenotype of G4OOV ER by increasing its ability to bind hormone. Overexpression of this protein also increases the activity of both wild type estrogen and glucocorticoid receptors (GR). The suppressor is the yeast homologue of the human p23 protein, a component of the molecular chaperonin complex bound to many unliganded steroid receptors.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA345446

Entities

People

  • Michael J. Garabedian

Organizations

  • NYU Langone Health

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Genes
  • Genetic Code
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Genome
  • Hormones
  • Identification
  • Laboratory Animals
  • Materials
  • New York
  • Phenotypes
  • Proteins
  • Suppressors

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology