Non-Invasive Detection of Axillary Nodes by Contrast Enhanced Magnetic Resonance Imaging.
Abstract
Previous studies in murine tumors have shown that the 3 drug combination of PALA + MMPR + 6AN PALA (n-phosphonacetyl aspartate), MMPR (6-methylmercaptopurine riboside), 6AN (6-aminonicotinamide); referred to as PMA is an effective radiation sensitizer. Using the MCF-7 human tumor, we have studied the effects of these drugs on metabolism and cell survival. In vivo studies demonstrate the presence of 6-phosphogluconate (6PG) and a reduction in nucleoside triphosphates after treatment with PMA. MCF-7 tumors were found to be more sensitive to radiation than previously studied murine tumors. PMA is more toxic to nude mice than to C3H mice but appears to be tolerable, although further studies are ongoing. In vitro studies with MCF-7 cells demonstrated that 6AN is not toxic by itself, but does induce metabolic inhibition, based on depletion of NTP and the appearance of 6PG. Ongoing in vivo studies include completion of the 31P NMR metabolic studies and tumor growth delay assays. In vitro studies include cell survival studies on the effect of 6AN in enhancing radiation response.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1997
- Accession Number
- ADA346801
Entities
People
- Jason A Koutcher
Organizations
- Memorial Sloan Kettering Cancer Center