Analysis of the Fission Yield Rad3+ Gene Product
Abstract
Schizosaccharomycespombe rad3(+) is homologous to the human ATM gene, which when mutated, causes the autosomal recessive human disease ataxia-telangiectasia. A-T patients suffer from a variety of symptoms, including a predisposition to cancers such as leukemias and lymphomas. A-T heterozygotes may also have an increased risk of developing cancers, in particular breast cancer. ATM and Rad3p are both structurally and functionally similar, as cells from A-T patients and rad3(-) yeast mutants both have sensitivities to ionizing radiation, and defects in cell cycle checkpoints. Since fission yeast Rad3p is believed to be involved in monitoring the state of the DNA, we have investigated genetic interactions between Rad3p and DNA replication proteins by using both genetic and biochemical approaches. We have also initiated a structure/function analysis of rad3(+), which has identified domains which appear to be important for normal rad3(+) function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1998
- Accession Number
- ADA349105
Entities
People
- Carolyn R. Chapman
Organizations
- Harvard Medical School