Genetic Analysis of Vertbrate Circadian Rhythmicity.

Abstract

The goal of this project was to develop technology needed to identify vertebrate circadian clock genes. An understanding of basic molecular mechanisms of biological clocks is important in designing treatments of the performance deficits, sleep disorders and other problems associated with jet lag, shift work, and organic circadian clock disorders in humans. A novel automated image analysis system was developed to measure circadian locomotor rhythms. This system was used to measure behavioral rhythms in zebrafish, a vertebrate organism that is useful for large scale mutagenesis screens. A screen for dominant mutations that alter the period of zebrafish locomotor rhythms was initiated, and 1500 animals with different mutagenized genomes were tested. The progeny of 40 putative mutants are being tested to determine whether they carry authentic clock mutations.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1998
Accession Number
ADA349604

Entities

People

  • Gregory M. Cahill

Organizations

  • University of Houston

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Animals
  • Circadian Rhythms
  • Computers
  • Data Acquisition
  • Data Analysis
  • Diseases And Disorders
  • Eukaryotes
  • Fish
  • Genes
  • Genetic Mapping
  • Genetic Phenomena
  • Genetics
  • Image Processing
  • Images
  • Mutations
  • Operating Systems
  • Video Images

Fields of Study

  • Biology
  • Psychology

Readers

  • Circadian Sleep-Wake Regulation and Chronobiology
  • Molecular Genetics

Technology Areas

  • Biotechnology