Development of Corneal Equivalents for In Vitro Testing of Ocular Irritants.
Abstract
Using tissue culture methods developed by our research team, we have achieved standardized methods for producing organotypic models of the rabbit and human cornea. One rabbit cornea will provide enough seed culture cells to produce 40-50 rabbit corneal equivalents. One human cornea will provide enough seed culture cells to produce 3-5 human corneal equivalents. No live animals were used in this research project as we obtained the rabbit eyes from a meat processing plant. Human corneal tissue was recovered from post-surgical specimens following corneal transplant surgery. Immunologic techniques were used to develop a reference biomarker database. Interleukin-1 (IL-i) was not detected in the rabbit or human native tissue or the rabbit corneal equivalents. intercellular adhesion molecule-1 (ICAM-1) and Fibronectin (FN) were inconsistently found in the human and rabbit native tissue and were not detected in the rabbit corneal equivalents. Heat Shock Protein 70 (HSP70) was consistently detected and cataloged in a reference database for both the human and rabbit native tissue and the rabbit corneal equivalents. Rabbit organotypic corneal models demonstrated characteristic histopathologic changes such as coagulative necrosis and liquefactive necrosis following in vitro exposure to prototypic chemicals.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 02, 1998
- Accession Number
- ADA349607
Entities
People
- Thomas E. Eurell
Organizations
- University of Illinois Urbana–Champaign