Immunotherapy of Congenital SIV Infection.

Abstract

During year 03, significant progress has been made in this project. Using the cost effective mouse model involving Rauscher murine leukemia virus (RLV), we have demonstrated that DNA vaccination can induce protection against virus challenge, even when the animals were vaccinated only with gag-pol expression vectors, indicating that neutralizing antibodies are not required for vaccine protection. A large DNA vaccine experiment involving neonatal rhesus macaques has been initiated at the Yerkes Regional Primate Research Center. The course of DNA inoculations has been completed, and all animals have been tested for humoral and cellular immune responses to the vaccines. Overall, 62.5% of the vaccines were seropositive for Env by ELISA, although no neutralizing antibodies were detected. None of the vaccinees had specific cytotoxic I-cell responses. Given these results, boosting with either recombinant gpi6O or additional DNA inoculations has been started.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1997
Accession Number
ADA349835

Entities

People

  • Ruth M. Ruprecht

Organizations

  • Dana–Farber Cancer Institute

Tags

DTIC Thesaurus Topics

  • Antigens
  • Blood
  • Cell Line
  • Cells
  • Chemistry
  • Culture Techniques
  • Dna Sequence Analysis
  • Drug Therapy
  • Health Services
  • Hiv Infections
  • Infection
  • Lymphatic System
  • Lymphocytes
  • Vaccines
  • Virus Diseases
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology