Preliminary Coupled Diffusion/Reaction Model for the Bioremediation of TNT Through the Use of Plant Enzymes
Abstract
Research at the Environmental Research Laboratory-Athens (ERL-Athens) on environmental transformations of nitroaromatic compounds has led to the isolation and characterization of a reductive protein from sediments and aquatic plants that readily reduces nitroaromatic compounds to the corresponding amino compounds. Under aerobic conditions, these nitroaromatic amines are readily oxidized to the catechols. The protein was isolated from sediments by extraction and purified by column chromatography using TNT reduction as an essay for protein activity. The purified protein was used to obtain the monoclonal antibody later used to determine the presence of this protein in solution. A field immunoassay was subsequently developed to help locate the source of protein.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1995
- Accession Number
- ADA350635
Entities
People
- L. J. Criscenti