Exploiting BCL-2 Overexpression in the Chemotherapy of Breast Cancer
Abstract
We have previously demonstrated the potentiation of apoptosis by overexpression of bcl-2 in pheochromocytoma cells in culture. Others have shown that estrogen treatment increases the expression of bcl-2 in MCF-7 human breast cancer cells. We have combined these findings in our hypothesis that estrogen treatment will result in increased bcl-2 expression and consequent increased susceptibility to neocarzinostatin-induced apoptosis in MCF-7 cells. Using both native MCF-7 lines and transfectants thereof, we have demonstrated the overexpression of bcl-2 in response to estrogen treatment. However, contrary to our expectations, overexpression of bcl-2 in these cells does not lead to enhancement of apoptosis induced by neocarzinostatin. Preliminary studies in our laboratory suggest that this deviation from prediction results from a difference in glutathione handling between breast cancer and neural cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1998
- Accession Number
- ADA350950
Entities
People
- Nina F. Schor