Cripto-1 in Mammary Gland Development and Carcinogenesis
Abstract
The purpose of this study is to evaluate the role of the growth factor Cripto-1 (CR-1) in mammary development and carcinogenesis. This report describes the progress during the first year of support. During this period, it was discovered that the ribozyme which had been synthesized and tested acted through a primarily non-catalytic mechanism. This finding has been published (Appendix 1), where it was shown that this hammerhead-like molecule, targeted to the extreme 5' end of the CR-1 message, was very effective at eliminating CR-1 expression, even without catalytic activity. Due to this finding, we have designed 2 additional ribozymes and are making some changes in the expression vectors. It was also determined that developing mammary epithelium can be transduced by an injected retrovirus, but that expression of the transgene only occurs in the terminal end buds. Finally, it was found that overexpression of CR-1 in preneoplastic CL-S1 cells did not affect their growth. Work during the next year will include refinement of the ribozyme strategy and further analysis of in vivo transduction. In addition, we will conduct more experiments with altered CR-1 expression in the mammary cell lines to clarify the findings discussed in this report.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1998
- Accession Number
- ADA352385
Entities
People
- Randall L. Kintner
Organizations
- Washington State University