Cell Culture Methods for the Selection of Osteoblast-Like Cells from the Periodontal Ligament
Abstract
Periodontal regenerative therapy involves restoration of lost periodontal architecture through the coordinated efforts of fibroblasts, osteoblasts and cementoblasts. The periodontal ligament plays an important role in this process because it is thought to contribute necessary progenitor cells for regeneration. Numerous reports indicate phenotypic heterogeneity among cells of the periodontal ligament. In addition to populations of cells exhibiting a fibroblast phenotype, cells with the capacity to form mineralized tissue have also been identified. A better understanding of these mineral-forming cells will contribute to the development of future regenerative therapies. The aim of this investigation was to use cell culture techniques to establish cell populations from human periodontal ligament enriched in the osteoblast-like phenotype. A second aim was to establish continuous periodontal ligament cell lines representative of both fibroblast-like and osteoblast-like cell types for future in vitro investigations.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1998
- Accession Number
- ADA353152
Entities
People
- Michael E. Poth
Organizations
- Air Force Institute of Technology