Characterization of Two Proteins which Interact with the BRCA1 Gene
Abstract
The functions and the intracellular localization of the breast/ovarian susceptibility gene product, BRCA1, has been controversial. To arrive at a clear understanding of its localization and relative position to other nuclear structures, a new monoclonal antibody was produced and characterized by immunohistochemical techniques with other BRCA1 antibodies. Each of the antibodies specifically detected BRCA1, in a variety of cells and in a cell cycle-dependent manner, localized to specific nuclear domains. However, all antibodies also cross-reacted with the centrosomal domain suggesting that BRCA1 is also localized to this important mitotic component. We found that the BRCA1 containing nuclear domains are different than any of the well defined nuclear domains. However, a cell cycle-related partial overlap was found for HP1a, a chromodomain-containing protein involved in heterochromatin maintenance. Cellular stimuli, such as heat shock and herpesvirus infection, dispersed BRCA1 from its domains. However, infection with Adenovirus 5 recruited BRCA1 to regions of viral transcription and replication. These disparate distributions of BRCA1 may provide clues to its function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1998
- Accession Number
- ADA353422
Entities
People
- Frank J. Rauscher
Organizations
- Wistar Institute