Molecular Mechanisms of Metastasis Suppression in Human Breast Cancer.
Abstract
The major cause of cancer deaths can be attributed to metastasis. Our goal is to identify metastasis- controlling genes for human breast cancer. This research is based upon our finding that microcell-mediated transfer of chromosome 11 into MDA-MB-43 5 results in nearly complete suppression of metastasis without suppressing tumorigenicity. The key findings during this reporting period were: (1) Kai-1 mRNA expression levels correlate with metastatic potential of a panel of human breast carcinoma cell lines; (2) transfection of Kai-1 partially suppresses metastasis of MDA-MB-435; (3) increased expression of PKC sigma correlates with metastatic propensity; and (4) transfection with MEK1 transforms N1H3T3 cells and makes them metastatic. Preliminary results include: (1) Using differential display, we also identified six novel differentially expressed genes in neo 11/435 hybrids compared to controls. Obtaining lull-length cDNAs and characterization are underway. We have also begun selection (3X) for enhanced lung colonization potential of MDA-MB-435
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA354066
Entities
People
- Danny R. Welch
Organizations
- Penn State College of Medicine