Regulation of Neu Signaling in Breast Cancer.
Abstract
ErbB2/neu amplification and overexpression is the most common genetic alteration in breast cancer. As a growth factor receptor and member of the EGFR family, ErbB2 signaling is dependent upon target tissue expression of ErbB2 regulating growth factors and other EGFR family members. To determine the role ErbB2 plays in normal and malignant mammary gland development I examined expression of ErbB2 agonists by ISH during normal development of the mouse mammary gland. This analysis has been completed for NRG1 and betaC. NRG1 is expressed within the stroma of both virgin and pregnant mammary glands. BetaC on the other hand is expressed within stroma of virgin glands and epithelial cells during pregnancy. This result suggests that betaC has differing functions during mammary gland development. To determine the role signaling by ErbB2 and ErbB4 plays in mammary gland development I expressed dominant negative forms of these receptors as a transgene within the developing mammary gland. These results indicate that ErbB2 is required for normal lobuloalveolar development, while both ErbB2 and ErbB4 play important roles in lactation at parturition. Thus these two receptors may be coupled at parturition.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA354785
Entities
People
- Frank Jones
Organizations
- Yale University