Identification of Two Candidate Tumor Suppressor Genes on Chromosome 17p13.3: Assessment of Their Roles in Breast and Ovarian Carcinogenesis.
Abstract
We have identified a region of less than 30 kbp, on chromosome 17p13.3 by allelic loss mapping, which is altered in >50% of the breast tumors analyzed. Using positional cloning techniques we have identified several genes, one which we refer to as OVCA2, that fall within this critical region. To date, we have found that: (1) OVCA2 is a new gene residing in a chromosomal region which is frequently lost in breast, brain, colon, ovarian tumors, acute myeloid leukemia and myelodysplastic syndromes, (2) OVCA2 is a secretory protein which is highly evolutionarily conserved, (3) OVCA2 (25 kDa) is proteolytically processed to its mature form (21 kDa), (4) p21 is present in normal blood serum and in breast nipple aspirate fluid, (5) both forms of OVCA2 are present in a variety of tissues, including mammary epithelium, (6) secretion of OVCA2 into the breast lumen is dependent on the developmental stage of the mammary gland, and (7) secreted OVCA2 appears to be lower in most breast tumors, relative to normal mammary epithelial cells. The recent detection of p21OVCA2 in normal serum and NAF, suggests that OVCA2 could be a new hormone with an important paracrine function. Identifying the potential role of OVCA2 in the development and the pathogenesis of the breast might ultimately help us to better understand the disease and to plan more effective treatment strategies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA355315
Entities
People
- Andrew K. Godwin
Organizations
- Fox Chase Cancer Center