Complement Activation by Liposome-Encapsulated Hemoblobin in Vitro: The Role of Endotoxin Contamination.

Abstract

Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37 deg C led to accelerated decay of serum complement (C) hemolytic activity (CH50). Empty liposomes (L) caused less expressed decrease of CH50, whereas free Hb had no effect on C hemolytic activity. The LEH- and L-induced increase in C consumption was unlikely due to endotoxin (LPS) contamination, as spiking of rat serum with LPS caused reduction in CH50 only at levels significantly higher than detectable in LEH or L. The LPS-induced C consumption was not potentiated by free hemoglobin.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1994
Accession Number
ADA355444

Entities

People

  • Alan S. Rudolph
  • Carl R. Alving
  • Janos Szebeni
  • Nabila M. Wassef

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Allergy And Immunology
  • Anaphylaxis
  • Biochemistry
  • Biomedical Research
  • Blood
  • Blood Cells
  • Cells
  • Chemical Compounds
  • Chemistry
  • Contamination
  • Diseases And Disorders
  • Endotoxins
  • Hemoglobin
  • Incubation
  • Materials
  • Rodents
  • Synthetic Membranes

Fields of Study

  • Biology
  • Medicine

Readers

  • Cardiovascular Physiology
  • Immunology