A Solid Support Synthesis and Novel Conjugation Methods of Breast Tumor Associated antigen: Toward the Development of Cancer Vaccines.
Abstract
Immunological study of Globo-H, a breast tumor associated carbohydrate antigen, has been producing promising results. As the demand for the supply of this antigen grows, the efficient production of enough material has become an urgent problem. We have been trying to solve this problem by two approaches: the solid support synthesis of Globo-H, and the development of efficient conjugation methods. For the solid support synthesis, solution phase direct roll-over reactions have been performed to verify the possibility that this method can be used to install the 2-amino-2-deoxy galactose moiety on the polymer support. From the results of these reactions, a tumor associated nonasaccharide antigen KM-i has been synthesized. To improve the conjugation yield of Globo-H to a carrier protein KLH (keyhole lympet hemocyanin), a model study has been carried out to implement an N-hydroxy succinimide ester conjugation method. At the same time, sufficient amounts of Globo-H have been synthesized by solution phase protocol for further immunocharacterization.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA356136
Entities
People
- Hyunjin Kim
- Samuel Danishefsky
Organizations
- Memorial Sloan Kettering Cancer Center