Role of the ATM Gene in Bilateral Breast Cancer Following Radiotherapy.
Abstract
The purpose of this project is to determine whether women who developed bilateral breast cancer following radiotherapy for an initial breast cancer had a higher incidence of heterozygosity for a mutation in the ATM gene than either breast cancer patients who did not develop a second breast cancer or bilateral breast cancer patients who did not receive radiotherapy. The approach has been to amplify with PCR the ATM gene in segments employing either cDNA produced using RNA isolated from blood lymphocytes or DNA extracted from paraffin-embedded biopsy tissue samples. During the first year of this project a total of 11 5 tissue samples from unilateral and bilateral breast cancer patients have been obtained, PCR products produced from these samples and examined for mutations using a Non-radioisotopic RNase Cleavage-based Assay (NIRCA). Analysis of the entire ATM coding region has been completed for 29 unilateral breast cancer patients and no mutations were identified. These results are consistent with the conclusion that the ATM mutation rate among unilateral breast cancer patients is no greater than the 0.5-1 % observed for the general population. Work is now in progress to perform this mutational analysis for bilateral breast cancer patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA356183
Entities
People
- Barry S. Rosenstein
Organizations
- Icahn School of Medicine at Mount Sinai