Structural and Functional Studies of Experimental HIV Synthetic Peptides Immunogens.
Abstract
Work performed in this grant continues to address major hurdles in the development of an effective synthetic peptide HIV vaccine: 1) the ability of synthetic peptides to mimic conformational epitopes within the HIV envelope protein and induce antibody responses capable of neutralizing primary HIV isolates and 2) the design of synthetic peptide immunogens capable of being recognized by MHC Class I and II molecules in outbred populations. In technical aim #1, computer analysis of the envelope protein sequence of CCR5-utilizing and CXCR4-utilizing HIV isolates has allowed us to synthesize novel HIV synthetic peptides that contain consensus sequences specific for CCR5-utilizing and CXCR4-utilizing viruses. One new peptide identified in aim #1, C4E9V-SP788- 89.6P, has induced sera capable of neutralizing SHIV-89.6 and the pathogenic SHIV- KB9 cloned virus. In technical aim #2, C4E9V-SP788-89.6P has been synthesized and prepared for structural studies. In technical aim #3, MHC Class I binding epitopes within the C4-V3 peptides have been identified for a variety of different HLA molecules.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA356192
Entities
People
- Barton Haynes
Organizations
- Duke University Hospital