The Antigenicity of Bacillus globigii Spores.

Abstract

Immunoassay of Bacillus globigii spores were carried out using a number of polyclonal antibody preparations. The limits of detection for the various antibody preparations gave similar values: all in the range of 1 ng or less. It was determined that, although spores reconstituted in distilled water could be detected in small amounts, the antibodies were directed at a soluble component in the samples, rather than the spores themselves. When the soluble component was separated from the spores by centrifugation, it was found that this component was responsible for over 90% of the immunoassay signal even though it composed less than 10% of the total weight. The nonreactivity of BG spores was also observed in transmission electron microscopy. Electron micrographs of the spores immunostained with colloidal gold did not indicate any appreciable antibody interaction.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1998
Accession Number
ADA356481

Entities

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  • Eric Kokko
  • Gail H. Thompson
  • William E. Lee

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  • Sensors

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  • Abstracts
  • Albumins
  • Biometric Security
  • Detection
  • Dilution
  • Electron Microscopes
  • Electron Microscopy
  • Filters
  • Filtration
  • Identification
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  • Materials
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  • Standards
  • Transmission Electron Microscopy

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  • Biology

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  • Agricultural Chemistry/Soil Science
  • Microbial Pathology
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  • Microelectronics