Use of p53 Mutation Analysis for Staging Breast Cancer

Abstract

This grant began with a collaboration between Oncormed and Dr. Timothy O'Leary from the Department of the Army. Dr. O'Leary was to provide the samples for the evaluation of the Molecular Staging Assay and its applications for detection of micrometastasis in breast cancer. The samples Dr. O'Leary provided were from a retrospective study. When these samples were collected, the protocols in use at the time were not compatible with retaining the genetic material intact. As a result, the DNA that was isolated to perform the Molecular Staging Assay was quite degraded. Oncormed extended a great deal of energy in attempts to salvage DNA of sufficient quality and quantity from these samples to do the study without success (see report from?). After it was decided that Dr. O'Leary's samples could not be used to complete this study, another collaborator was identified. Dr. Bruce Ponder from ? had 100 samples from breast cancer patients that could be used to complete this study. An initial evaluation of the samples indicated they were of sufficient quality and quantity. The work was initiated on these samples and a summary of the progress is attached.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1998
Accession Number
ADA357313

Entities

People

  • Marga White

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Acquisition
  • Alkanes
  • Animals
  • Attachment
  • Biomedical Research
  • Breast Cancer
  • Contracts
  • Costs
  • Deoxyribonucleic Acids
  • Fringe Benefits
  • Indirect Costs
  • Laboratory Animals
  • Materials
  • Mutations
  • Neoplasms
  • Recombinant Dna

Readers

  • Military History
  • Oncology and Biomarker-Based Cancer Detection.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology