Role of HMG-I (I) in Human Breast Cancer Metastasis
Abstract
HMG-I(Y) proteins are mammalian architectural transcription factors that regulate transcription of a number of genes. The original proposal is to test a hypothesis that expression of HMG-I(Y) proteins will fundamentally influence the expression of genes, which are involved in metastatic invasion, migration, angiogenesis and colonization. In the second year of study, a great effort has been made to isolate HMG-I(Y) high-expressing breast cancer cell transfectants. These HMG-I(Y) transfectants and HMG-I(Y) monitoring systems are essential tools for further study of metastatic potential and expression of down-stream genes in HMG-I(Y) transfectants. Two new approaches have been developed. (a) HA-tagged HMG-I(Y) gene constructs were generated. Isolation of stable HMG-I(Y)-expressing clones, including dominant negative HMG-I(Y) clones, by using monoclonal antibody against HA-tag is in progress. (b) HA-tagged HMG-I(Y) genes were subcloned into a tetracycline inducible gene expression system. The preliminary results indicated that the exogenous HMG-I(Y) proteins in MCF-7 cells could be increased to 50-fold after tetracycline induction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA357366
Entities
People
- Ying Li
Organizations
- Washington State University