Dual-Specificity Anti-HER-2/neu Antisense DNA Agents for Breast Cancer Therapy.
Abstract
The purpose of the project is to develop a new class of antisense drug and a procedure for selecting an optimal antisense compound with HER-2/neu mRNA as the target. The scope for this year includes preparation of HER-2/neu target RNA (Task 1), development of a novel peptide synthesis technology for generation of combinatorial libraries (Task 2) and preliminary experiments on identifying members of a library that bind HER-2/neu RNA (Task 3). Task 1 has been somewhat modified in that one representative RNA fragment has been prepared, but this fragment has been further analyzed and found to have sensitive RNase H cleavage sites, which is a highly favorable result. Task 2 has been successfully completed in that a protocol for peptide synthesis in solution phase on disulfide-linked polyethylene glycol has been developed. Task 3 (months 9-12) has not yet been initiated due to unanticipated personal problems.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA357699
Entities
People
- Stanely Stein