Complementation Screening in Mammalian Cells: Application to Cell Immortalization.
Abstract
The broad goals of my initial proposal were two-fold. First, I proposed the development of a genetic system that would allow complementation screening in animal cells much in the way that this is done in yeast. Second, I proposed the application of this approach to problems of mortality control and tumorigenesis in breast tumor cells and their normal precursors. As a model system, I chose primary, normal human mammary epithelial cells (HMEC). Progress over the last year can be summarized as follows: (1) Development and validation of the genetic system has been largely completed. (2) We have determined that the lifespan of HMEC cells can be altered by manipulation of telomere length. (3) We have found that a commonly activated cellular oncogene, c-myc, can regulate telomerase activity in HMEC cells and in normal fibroblasts. (4) Either myc expression or telomerase activation can effectively immortalize HMEC cells. (5) We have expanded our genetic analysis of neoplastic transformation to include other aspects of the process, namely resistance to growth inhibitory cytokines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA357868
Entities
People
- Gregory Hannon