Genes Altered by Intracisternal A Particles in Mouse Mammary Tumorigenesis.
Abstract
A mouse cDNA library constructed from a DMBA-induced primary mammary tumor which expressed high levels of intracisternal. A particles was screened by differential hybridization using probes from the LTR and body of the element to find genes chimeric with IAPs. The 3' end of a gene, kokopelli, was isolated which showed tumor specific expression. The gene demonstrated tumor specific expression in all mouse mammary tumors and hyperplastic and neoplastic cell lines. Northern analysis of clones isolated from normal kidney, liver and brain mouse cDNA libraries suggested isolation of the normal counterpart to the tumor specific gene. Sequence analysis of these clones, however, revealed no significant homology outside a common B1 repetitive element. The cDNA clone from the kidney was localized to mouse chromosome 7 by genetic backcross analysis. Kokopelli was isolated as a chimera with U1 snRNP specific protein C. This association was further confirmed by isolation of 3' RACE products from a D2 tumor and from normal mammary gland. Further attempts to isolate the end of the 5' tumor specific transcript as well as the normal counterpart continue.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA357907
Entities
People
- Michael R. Crowley
Organizations
- Health Research, Incorporated