The Role of Prostaglandin G/H Synthase 2 in the Apoptosis of Human Mammary Epithelial Cells.
Abstract
We are examining apoptotic pathways that may be altered in human mammary epithelial cells as a consequence of the overexpression of prostaglandin G/H synthase 2. This project involves quantifying the apoptotic response as well as apoptotic regulators (ATM, MDM2, p53, bcl-2, and bax) in this cell system following exposure to agents that can induce apoptosis. We have shown that human mammary epithelial cells express high levels of prostaglandin G/H synthase, are resistant to ionizing radiation-induced apoptosis, but do apoptose when exposed to the broad spectrum kinase inhibitor staurosporine. This report documents progress made in the past year in measuring the apoptotic response in human mammary epithelial cells, in measuring the protein levels of some apoptotic regulators, and in the creation of human mammary epithelial cells that have attenuated levels of prostaglandin G/H synthase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1998
- Accession Number
- ADA357964
Entities
People
- Robert Fisher
Organizations
- University of North Carolina at Chapel Hill