Soy Metabolites, Isoflavones in Cell Growth and Apoptosis.
Abstract
The hypothesis to be tested in this proposal was that soy metabolites selectively eliminate aberrant breast epithelial cells (tumor cells) by altering the expression of specific cell cycle regulatory genes which, in turn, causes cell cycle arrest and induces apoptosis. Tumors often occur as a result of uncontrolled and deregulated cell cycle caused by an imbalance of CKIs and CDKs. Current knowledge dictates that the deregulation of many classes of genes are involved in tumorigenesis, such as oncoproteins, tumor suppressors, signaling pathways, growth factors or cytokines, apoptosis inducers and potentiators (1). Protectors and promoters of apoptosis interact with each other, and the outcome of this interaction, apoptosis or survival, depends on the ratio of the death promoters to the death suppressors (2). These apoptosis regulators are part of and interact with the cell cycle machinery. Therefore, the purpose of this study is to explore the mechanism of the soy metabolites, genistein and daicizein, in breast epithelial cells by examining any changes in the levels of cell cycle regulatory proteins induced or inhibited by these metabolites. We will investigate whether the observed changes in specific gene expression are consistent with apoptosis in breast cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA358055
Entities
People
- Fazlul Sarkar
Organizations
- Wayne State University