P/CAF Function in Transcriptional Activation by Steroid Hormone Receptors and Mammary Cell Proliferation.
Abstract
Inappropriate gene expression and abnormal progression of the cell cycle are intimately linked to oncogenesis and tumor formation. The purpose of this proposal is to examine the role of the PCAF histone acetyltransferase as a co-factor for steroid hormone receptors in the regulation of gene expression. Five specific aims define the scope of our project: 1) to complete the isolation and cloning of mouse PCAF cDNA and genomic sequences 2) to characterize PCAF as a histone acetyltransferase 3) to examine PCAF interactions with specific steroid receptors 4) To examine whether acetyltransferase activity is required for transcriptional activation by steroid hormone receptors 5) to create mice deficient for PCAF and mice that overexpress this factor to assess the role of PCAF in mouse development, differentiation, or tumorigenesis. In the past year we have largely completed the first and second aims of our project and have made good progress towards experiments described in the fifth specific aim as well. We have also discovered that another protein, GCN5 is highly similar to PCAF in both structure and acetyltransferase functions. We conclude that both proteins are important transcriptional regulators and will expand our aims to include studies of GCN5 as well as PCAF in steroid hormone responses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA358082
Entities
People
- Sharon Y. Roth
Organizations
- University of Texas at Austin