Role of Ser-Arg Proteins in the Regulation of RNA Processing.
Abstract
Deregulation of alternative splicing has been linked to malignant transformation in breast cancers. Therefore, to fully understand breast cancer, it will be important to identify and characterize factors that regulate the splicing process. We have previously identified a complex of nuclear matrix proteins related to Serine/Arginine- repeat (SR) splicing factors which is required for the splicing of specific pre-niRNAs (Blencowe et al., 1998). This complex contains the SR-matrix proteins of l6OkDa and 3OOkDa (SRml6O and SRm3OO) which, unlike proteins of SR family of splicing factors, lack RNA Recognition Motifs (RRMs). This complex associates with pre-mRNA through multiple interactions involving SR family proteins and small nuclear ribonucleoprotein particles (snRNPs). During the past year, we have completed the sequencing of SRm3OO and have investigated the function of the SRml6O/3OO complex in regulated splicing. We have demonstrated that SRml6O/3OO is required for a purine-rich exonic splicing enhancer (ESE) to promote the splicing of a pre-mRNA containing a weak 3' splice site.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA358213
Entities
People
- Benjamin J. Blencowe
Organizations
- Massachusetts Institute of Technology