The Molecular Basis of the Response to Radiation..
Abstract
The goal of this project is to further define at a molecular level the human gene products required for the normal 02 checkpoint response. The checkpoint response is a fundamental mechanism by which cells control their cell division cycle after experiencing DNA damage from radiation. This response results in an arrest in the G2 phase of the cycle until damage is repaired. We are exploiting the conservation of this pathway among eukaryotes to isolate human checkpoint genes by large-scale complementation screens isolating novel human cDNAs which can complement yeast G2 checkpoint mutant strains. In the first year of this award we have identified several new human cDNAs which are potential novel human checkpoint genes. In other Technical Objectives we have developed a panel of reagents from normal and malignant human breast cancer cell lines to determine the structure and expression of these genes in both normal and malignant mammary cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA358459
Entities
People
- Sharon E. Plon
Organizations
- Baylor College of Medicine