Nucleoprotein-Based Nanoscale Fabrication.
Abstract
We have provided a clear demonstration of each of the essential scientific principles governing this approach to macromolecular assembly. First, we have shown that the coupling reaction between methyltransferases and 5-FdC in DNA provides a mechanism for stable attachment of methyltransferases and chimeric methyltransferase fusion proteins in a pre-selected arrangement along a DNA molecule. In the course of this work we discovered that the FdC dramatically slows the rate of methyl transfer catalyzed by DNA methyltranserases. Slowing of the methyltransferase reaction by FdC could indicate aberrant hydrogen bonding between the fluorine atom on cytosine and groups at the enzyme active site. Alternatively the electron withdrawing power of the fluorine atom may diminish the capacity of the activated intermediate to attack the methyl group on the S- adenosyl-methionine methyl donor. Electronic structure calculations, at the Hartree-Fock Level of theory with the 6-31G* basis set, support the latter possibility. The calculated energy of the Highest Occupied Molecular Orbitals for models of all the possible intermediates at this stage of the reaction is significantly lowered by the presence of the 5-fluorine atom.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1998
- Accession Number
- ADA358464
Entities
People
- A. Laayoun
- D. J. Baker
- D. S. Joy
- J. A. Wendel
- L. Niu
- Stevens S. Smith
Organizations
- City of Hope National Medical Center