Preventive Role of Specific Dietary Factors and Natural Compounds Against DNA Damage and Oxidative Stress.

Abstract

This project is examining the hypothesis that certain antioxidant vitamins (C and E) and natural compounds (caffeic acid phenethyl ester CAPE and epigallocatechin gallate EGCG may be protective against mammary carcinogenesis, at lest in part, by decreasing the extent of DNA damage in the target tissue. This DNA damage may be caused by chemical carcinogens in the environment (e.g., benzo(a)pyrene) and/or dietary factors that increase endogenous production of reactive oxygen species. We are developing sensitive monoclonal antibodies and assays for measuring the oxidative DNA damage, 5-hydroxymethyldeoxyuridine (5HMU). Several hybridomas have been developed. A pilot study has investigated the effects of CAPE treatment on benzo(a)pyrene-DNA adducts in MCF-7 cells. A dose-dependent decrease in DNA damage with increasing CAPE concentration was observed indicating that the immunologic method is appropriate for these types of studies and supporting the original hypothesis.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1998
Accession Number
ADA358878

Entities

People

  • Shunji Morita

Organizations

  • Columbia University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Albumins
  • Animals
  • Antibodies
  • Antioxidants
  • Biological Products
  • Carcinogens
  • Cells
  • Culture Techniques
  • Materials
  • Molecules
  • Neoplasms
  • New York
  • Oxidative Stress
  • Pilot Studies
  • Proteins
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

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