Effect of Estrogen on Progression of Human Proliferative Breast Cancer Disease in a Xenograft Model

Abstract

Utilizing the T24-Ha-ras transfected MCF10A xenograft model of early human breast cancer progression we have: (1) demonstrated the direct effects of estrogen on growth and sequence of progression of human preneoplastic breast disease, (2) demonstrated the utility and validity of bcl-2, cyclin D1, and c-erbB2 as specific markers for breast cancer progression, and (3) established an in vitro assay system that recapitulates in vivo growth and organization of MCF10AT cells into highly proliferative estrogen responsive ductular elements. Experiments analyzing chemotherapeutic and chemopreventive effects of tamoxifen on growth and progression of MCF10AT lesions are underway. Our data thus far show that transcriptional activation of estrogen receptor gene is not a result of alteration in methylation status of estrogen receptor gene or a position effect of insertion of exogenous T24 Ha-ras gene.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1998
Accession Number
ADA359206

Entities

People

  • P. V. Shekhar

Organizations

  • Barbara Ann Karmanos Cancer Institute

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Confocal Microscopy
  • Culture Techniques
  • Data Analysis
  • Environmental Health
  • Genetics
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Proteins
  • Three Dimensional
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology