Explosives Conjugation Products in Remediation Matrices: Interim report no. 2

Abstract

Immobilization of 2,4,6-trinitrotoluene (TNT) in soils and in compost had been widely documented. Dramatic reductions in solvent extractable TNT have been observed, while complete recoveries of radioactivity from carbon-14 labeled TNT indicated that all of the products of TNT remained in the matrix in some form. The objectives of these studies were to determine the nature and mechanism of immobilization and the long-term stability, biodegradability, and toxicity of the final products. The approach included 15N-nuclear magnetic resonance analysis of binding to humic materials, acid/base hydrolysis with subsequent identification of products, microbial degradation, and toxicity studies. Results confirm covalent bonding of the amino transformation products of TNT that is only slightly reversible, depending upon the degree of completion to which the reactions have occurred. Products were resistant to microbial degradation and relatively stable against hydrolysis. Toxicity was related to bioavailability, which depended upon the degree of completion of the immobilization reactions. These results have important implication for fate of TNT in remediation systems and in the environment.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1998
Accession Number
ADA359387

Entities

People

  • D. Gunnison
  • H. Fredrickson
  • J. C. Pennington
  • L. S. Inouye
  • V. A. Mcfarland

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Chemical Analysis
  • Chemical Synthesis
  • Chemistry
  • Environment
  • Liquid Chromatography
  • Materials
  • Organic Chemistry
  • Surface Plasmon Resonance
  • Toxicity

Fields of Study

  • Environmental science

Readers

  • Agricultural Chemistry/Soil Science

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation