The In Vivo DNA Binding Properties of Wild-type and Mutant p53 Proteins in Mammary Cell Lines During the Course of Cell Cycle.
Abstract
Using centrifugal elutriation we have separated exponentially growing ML-1 cells into specific cell cycle populations and have treated these discrete fractions with Camptothecin for 6 hours. These experiments allowed us to determine that Camptothecin-induced apoptosis and activation of p53 Via stabilization and p53 phosphorylation occur in all stages of the cell cycle. We have also determined that p53 mediated activation of the mdm2 gene occurs from a constitutively nucleosome free region. Additionally we have observed that differential activation of p53 induced genes does not occur at the level of chromatin reorganization. Finally we have observed that mutant p53 and Spl can be coordinately purified from an Spl DNA affinity column.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA360032
Entities
People
- Erwin Fleissner
- Jill Bargonetti
Organizations
- Hunter College