Infusion of Autologous Lysed Plasma Into the Baboon: Assessment of Coagulation, Platelet, and Pulmonary Function.
Abstract
Autotransfusion of shed blood, cardiopulmonary bypass and thrombolytic therapy are all conditions known to elicit a fibrinolytic state in man, with generation of plasmin and production of degradation products of fibrinogen and fibrin. The role of plasmin and of these degradation products in producing the coagulopathy, platelet dysfunction and pulmonary dysfunction sometimes seen in these states remains highly controversial. Eight baboons were infused with 250 cc of autologous plasma treated with urokinase in doses of 3000 IU/ml or 5000 IU/ml, creating a fibrinolytic state in which plasmin was generated and fibrinogen and fibrin were cleaved. Aprotinin (1000 KIU/ml) was subsequently added to inhibit plasmin. This state resulted in a transient increase in activated partial thromboplastin time without a similar increase in thrombin time or prothrombin time, and a marked prolongation of the bleeding time lasting at least 24 but less than 48 hours. These changes may be attributed to the effects of FDP and D-Dimer or, alternatively, to the decrease in fibrinogen. No untoward effects of this state were observed clinically. No change in pulmonary or renal function was seen.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1996
- Accession Number
- ADA360134
Entities
People
- D. S. Morse
- G. Giorgio
- G. P. Cassidy
- G. R. Valeri
Organizations
- Boston University