Leukocytes Mediate Acid Aspiration Induced Multi-Organ Injury.
Abstract
Acid aspiration leads to lung injury characterized by neutrophil (PMN) sequestration and edema. This study investigates whether localized acid aspiration triggers both local and remote PMN sequestration and whether these cells are responsible for pulmonary edema and systemic organ injury. Rats pretreated with IV saline or rendered neutropenic underwent tracheostomy and insertion of a cannula into a lung segment. This was followed by instillation of either 0.1 N HCl or saline. After 30 min leukopenia was noted and circulating PMN produced H2O2. These activated cells were also primed to a second oxidative stimulus. In addition, 30 min after aspiration PMN were noted to be progressively sequestered in the non-aspirated lung as well as in the heart and kidney. This preceded the increased permeability and edema that developed in the lungs and systemic organs. At 3 h a correlation was found between the sequestered PMN and the increase in wet/dry ratio (W/d) of the non-aspirated lung (r = 0.82, p<0.05). In neutropenic rats there was a significant (p<0.05) reduction of localized aspiration induced: plasma thromboxane B2 and leukotriene B4 synthesis; generalized increase in lung W/d; and protein level in bronchoalveolar lavage of the aspirated and non-aspirated lungs. Neutropenia also lowered aspiration induced myeloperoxidase activity in the heart and kidney along with W/d of these organs (all p<0.05). These data indicate that localized acid aspiration activates circulating neutrophils and promotes their sequestration in the lungs and systemic organs. These cells are largely responsible for the multi-system organ injury.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 17, 1991
- Accession Number
- ADA360196
Entities
People
- C. R. Valeri
- Gideon Goldman
- Joseph M. Klausner
- Lester Kobzik
- Richard Welbourn
Organizations
- Boston University