Effects of Storage of CPD-ADSOL Red Cells at 4C for as Long as 49 Days, Biochemical Modification, Freeze-Preservation, and Post-Wash Storage at 4C for 24 Hours
Abstract
Human CPD-collected red blood cells from 7 healthy male volunteers were stored in ADSOL (adenine, glucose, mannitol, and sodium chloride solution) at 4C for various periods: the red blood cells from one volunteer were stored for 35 days; red blood cells from 3 volunteers were stored for 42 days; and red blood cells from 3 other volunteers were stored for 49 days. A 100 ml aliquot was removed from each unit for in vitro and in vivo survival measurements. The remainder of the unit was biochemically modified with pyruvate, inosine, phosphate, and adenine (PIPA) solution, frozen with 40% W/V glycerol using a double centrifugation procedure and stored at -80C for 84 to 309 days. After thawing and washing, the previously frozen red blood cells were stored at 4C for 24 hours prior to in vitro and in vivo testing. CPD-ADSOL red blood cells stored at 4C for up to 42 days, biochemically treated with PIPA solution, frozen, thawed, washed and stored at 4C for 24-hours exhibited a mean in vitro recovery value of 83%, a mean 24-hour posttransfusion survival value of 72%, improved oxygen transport function, and only minimal residual hemolysis. CPD-ADSOL red blood cells treated in an identical manner except for a 49-day liquid storage period had a mean in vitro recovery value of only 73% and a mean 24-hour posttransfusion survival value of only 61%, but ATP, DPG, P50 levels, and the level of residual hemolysis were satisfactory. Our data show that 42-days is the maximum period that red blood cells can be stored in CPD-ADSOL solution at 4C and have satisfactory in vitro recovery and in vivo survival results following biochemical treatment with PIPA solution, freezing, thawing, washing, and post-wash storage at 4C for 24-hours.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 27, 1992
- Accession Number
- ADA360302
Entities
People
- A. Giorgio
- A. X. Gray
- C. R. Valeri
- G. Ragno
- L. E. Pivacek